骨质疏松小鼠模型|BioMice百奥动物

骨质疏松症被定义为一种全身性骨骼疾病，其特征是骨量低和骨组织微观结构恶化，从而增加骨脆性和骨折易感性^[1]。骨质疏松症的基本发病机制是骨形成和吸收的失调。去卵巢小鼠是公认的人类绝经后骨质疏松症（PMOP）的体内模型。骨密度（BMD）测量、显微CT分析和组织形态计量学分析用于评估OVX小鼠^[2,3]。手术后采集骨骼并进行测试。百奥赛图在C57BL/6小鼠中建立了稳定的OVX诱导的骨质疏松症模型方案，可用于骨质疏松症的临床前研究和药效学评价。

骨质疏松模型构建及验证

OVX induced weight loss of body and uterus in mice

OVX induced bone loss in mice (CT)

Ovariectomy induced osteoporosis. C57BL/6 mice received ovariectomy on day 0. Six weeks after operation, bones and uterus were harvested and tested. Body weight were recorded everyday. Ovariectomy produced a bone loss in mice. Values are expressed as mean ± SEM. n=8, T-test, * p<0.05, **p<0.01.

OVX induced bone loss in mice (H&E)

Pathological analysis of ovariectomy-induced osteoporosis. Six weeks after ovariectomy, the bone trabecula area was decreased in mice that received OVX surgery. Values are expressed Values are expressed as mean ± SEM. n=8, T-test, * p<0.05, **p<0.01.

References

1. Eastell R, O‘Neill TW , Hofbauer LC et al. Postmenopausal osteoporosis. Nat Rev Dis Primers. 2016, 2:16069.

2. Wang HC, Zhou KF, Xiao FZ et al. Identification of circRNA-associated ceRNA network in BMSCs of OVX models for postmenopausal osteoporosis. Sci Rep. 2020, 10(1):10896.

3. Chen K, Qiu PC, Yuan Y et al. Pseurotin A Inhibits Osteoclastogenesis and Prevents Ovariectomized-Induced Bone Loss by Suppressing Reactive Oxygen Species. Theranostics. 2019, 9(6):1634-1650.

参考文献

1. Eastell R, O‘Neill TW , Hofbauer LC et al. Postmenopausal osteoporosis. Nat Rev Dis Primers. 2016, 2:16069.
2. Wang HC, Zhou KF, Xiao FZ et al. Identification of circRNA-associated ceRNA network in BMSCs of OVX models for postmenopausal osteoporosis. Sci Rep. 2020, 10(1):10896.
3. Chen K, Qiu PC, Yuan Y et al. Pseurotin A Inhibits Osteoclastogenesis and Prevents Ovariectomized-Induced Bone Loss by Suppressing Reactive Oxygen Species. Theranostics. 2019, 9(6):1634-1650.