Establishment of Inflammatory Bowel Disease Mouse Model
Experimental Animals：C57BL/6N, 7-10 weeks old, female
Detection Indicator：Body Weight, Colon Weight, Colon length
Modeling reagent：TNBS (trinitrobenzene sulfonate)
Efficacy Validation on IBD(wild-type C57BL/6) Mouse Model for CD4-GK1.5-mlgG2a
Effects of CD4-GK1.5-mlgG2a on TNBS-induced acute intestinal inflammation. Animals presensitizated with TNBS solution to the shaved skin area on day 0, and then received TNBS intrarectal administration on day 7. Body weight were recoded every day. CD4-GK1.5-mIgG2a (3, 10 mg/kg, i.p.) were injected on day 6. Mice were sacrificed on day 10 and colon weight (A) and length (B) were record, colon weight，body ratio were calculated (D-F).
Establishment of IBD Mouse Model
Percentage of initial body weight, clinical score and stool consistency of IBD mouse model were recoded every day.
Gross Sampling and Histologic Assessment of colon of IBD model
Representative pictures of gross anatomy and ratio of colonic weight and length of IBD mice. Values are expressed as mean ± SEM. **p<0.01.
Representative pictures of intestinal pathological tissue section of CD4+CD45RBhigh T cell -induced IBD model.