B-Tg(mEpha4) MC38|BioMice百奥动物

Common name	B-Tg(mEpha4) MC38	Catalog number	321873
Aliases	rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The exogenous promoter and mouse Epha4 coding sequence were inserted into the mouse genome randomly. Mouse Epha4 is highly expressed on the surface of B-Tg(mEpha4) MC38 cells.

Application

B-Tg(mEpha4) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-Tg(mEpha4) MC38 cells. The exogenous promoter and mouse Epha4 coding sequence were inserted into the mouse genome randomly.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mEpha4) MC38 cells. B-Tg(mEpha4) MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Tg(mEpha4) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.