B-Tg(mAreg) MC38|BioMice百奥动物

Common name	B-Tg(mAreg) MC38	Catalog number	321898
Aliases	AR, Mcub, Sdgf	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The exogenous promoter and mouse Areg coding sequence were inserted into the mouse genome randomly. Mouse Areg can be secreted by B-Tg(mAreg) MC38 cells.

Application

B-Tg(mAreg) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-Tg(mAreg) MC38 cells. The exogenous promoter and mouse Areg coding sequence were inserted into the mouse genome randomly.

Protein expression analysis

Areg expression analysis in B-Tg(mAreg) MC38 cells by ELISA. Mouse Areg was detected in the supernatant of B-Tg(mAreg) MC38 cells. The 1-D01 clone of B-Tg(mAreg) MC38 cells was used for in vivo experiments

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mAreg) MC38 cells. B-Tg(mAreg) MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Tg(mAreg) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.