B-hROR1 CT26.WT
|
Common name |
B-hROR1 CT26.WT | Catalog number | 322263 |
| Aliases | NTRKR1, dJ537F10.1 | Disease | Colon carcinoma |
|
Organism |
Mouse |
Strain | BALB/c |
| Tissue types | Colon | Tissue | Colon |
模型验证
Description
The mouse Ror1 gene was replaced by human ROR1 coding sequence in B-hROR1 CT26.WT cells. Human ROR1 is highly expressed on the surface of B-hROR1 CT26.WT cells.
Application
B-hROR1 CT26.WT cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Targeting strategy
Protein expression analysis
Gene targeting strategy for B-hROR1 CT26.WT cells. The exogenous promoter and human ROR1 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Ror1 gene, resulting in a non-functional transcript.
ROR1 expression analysis in B-hROR1 CT26.WT cells by flow cytometry. Single cell suspensions from wild-type CT26.WT and B-hROR1 CT26.WT cultures were stained with species-specific anti-ROR1 antibody. Human ROR1 was detected on the surface of B-hROR1 CT26.WT cells but not wild-type CT26.WT cells.
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