B-hMUC1 MC38|BioMice百奥动物

Common name	B-hMUC1 MC38	Catalog number	311266
Aliases	MUC1, CD227, PEM, EMA, PUM, KL-6, MAM6, MCKD, PEMT, H23AG, MCKD1	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Muc1 gene was replaced by human MUC1 coding sequence in B-hMUC1 MC38 cells. Human MUC1 is highly expressed on the surface of B-hMUC1 MC38 cells.

Application

B-hMUC1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. It should be noted that B-hMUC1 MC38 cells can grow on B-hMUC1 mice, but can’t grow on wild-type C57BL/6 mice, which is consistent with the literature report.

Targeting strategy

Gene targeting strategy for B-hMUC1 MC38 cells. The exogenous promoter and human MUC1 coding sequence was inserted to replace murine exon 1~7. The insertion disrupts the endogenous murine MUC1 gene, resulting in a non-functional transcript.

Protein expression analysis

MUC1 expression analysis in B-hMUC1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hMUC1 MC38 cultures were stained with species-specific anti-MUC1 antibody. Human MUC1 was detected on the surface of B-hMUC1 MC38 cells but not wild-type MC38 cells. The 2-C07 clone of B-hMUC1 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hMUC1 MC38 cells. B-hMUC1 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into B-hMUC1 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hMUC1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hMUC1 MC38 cells were subcutaneously transplanted into B-hMUC1 mice (n=6), and on 31 days post inoculation, tumor cells were harvested and assessed for human MUC1 expression by flow cytometry. As shown, human MUC1 was highly expressed on the surface of tumor cells. Therefore, B-hMUC1 MC38 cells can be used for in vivo efficacy studies of novel MUC1 therapeutics.