B-hHER2 MC38|BioMice百奥动物

Common name	B-hHER2 MC38	Catalog number	310701
Aliases	ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2, erb-b2 receptor tyrosine kinase 2	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Erbb2 gene was replaced by human ERBB2 coding sequence in B-hHER2 MC38 cells. Human ERBB2 is highly expressed on the surface of B-hHER2 MC38 cells.

Application

B-hHER2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hHER2 MC38 cells.The exogenous CAG promoter and human ERBB2 coding sequence was inserted to replace part of murine exon 2 and all of exons 3-7. The insertion disrupts the endogenous murine Erbb2 gene, resulting in a non-functional transcript.

Protein expression analysis

HER2 expression analysis in B-hHER2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hHER2 MC38 cultures were stained with species-specific anti-HER2 antibody. Mouse HER2 was detectable in wild-type MC38 cells. Human HER2 was detected on the surface of B-hHER2 MC38 cells but not wild-type MC38 cells. The 2-B06 clone of B-hHER2 MC38 cells was used for in vivo tumor growth assays.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHER2 MC38 cells. B-hHER2 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHER2 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Cell Line Inoculation Precautions:

Inoculated cell lines can be suspended with DMEM stock solution.

Before implementing the project, it is recommended to do tumor growth experiments. The cell inoculation amount is between 1E6-1E7.

Protein expression analysis of tumor cells

B-hHER2 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5) . At the end of the experiment, tumor cells were harvested and assessed for human HER2 expression by flow cytometry. As shown, human HER2 was highly expressed on the surface of tumor cells. Therefore, B-hHER2 MC38 cells can be used for in vivo efficacy studies of HER2 therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHER2 MC38 cells. B-hHER2 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into heterozygous B-hCD3E/hHER2 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHER2 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Cell Line Inoculation Precautions:

Inoculated cell lines can be suspended with DMEM stock solution.

Before implementing the project, it is recommended to do tumor growth experiments. The cell inoculation amount is between 1E6-1E7.

Protein expression analysis of tumor cells

B-hHER2 MC38 cells were subcutaneously transplanted into heterozygous B-hCD3E/hHER2 mice (female, 7-week-old, n=5), and on 31 days post inoculation, tumor cells were harvested and assessed for human HER2 expression by flow cytometry. As shown, human HER2 was highly expressed on the surface of tumor cells. Therefore, B-hHER2 MC38 cells can be used for in vivo efficacy studies of HER2 therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHER2 MC38 cells. B-hHER2 MC38 cells (5x10⁵, 1x10⁶, 5x10⁶) were subcutaneously implanted into homozygous B-hCD3E mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHER2 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Cell Line Inoculation Precautions:
Inoculated cell lines can be suspended with DMEM stock solution.
Before implementing the project, it is recommended to do tumor growth experiments. The cell inoculation amount is between 1E6-1E7.

Protein expression analysis of tumor cells

B-hHER2 MC38 cells were subcutaneously transplanted into B-hCD3E mice (n=5), and on 35 days post inoculation, tumor cells were harvested and assessed for human HER2 expression by flow cytometry. As shown, human HER2 was highly expressed on the surface of tumor cells. Therefore, B-hHER2 MC38 cells can be used for in vivo efficacy studies of HER2 therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHER2 MC38 cells. B-hHER2 MC38 cells (5x10⁵, 1x10⁶, 5x10⁶) were subcutaneously implanted into homozygous B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHER2 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHER2 MC38 cells. B-hHER2 MC38 cells (5x10⁵, 1x10⁶, 5x10⁶, 1x10⁷) were subcutaneously implanted into homozygous B-h4-1BB/hHER2 mice (female, 11-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHER2 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Cell Line Inoculation Precautions:

Inoculated cell lines can be suspended with DMEM stock solution.

Before implementing the project, it is recommended to do tumor growth experiments. The cell inoculation amount is between 1E6-1E7.