||B-hHER2 EL4||Catalog number||310724|
|Aliases||CD340, HER-2, ERBB2||Disease||Lymphoma|
|Tissue types||Lymphoma||Tissue||T lymphocyte|
The mouse Erbb2 gene was replaced by human ERBB2 coding sequence in B-hHER2 EL4 cells. Human HER2 is highly expressed on the surface of B-hHER2 EL4 cells.
B-hHER2 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Gene targeting strategy for B-hHER2 EL4 cells. The exogenous promoter and human ERBB2 coding sequence was inserted to replace part of murine exon 2 and all of exons 3~7. The insertion disrupts the endogenous murine Erbb2 gene, resulting in a non-functional transcript.
Protein expression analysis
Animals with tumor volume over 3000mm3 in the control group were euthanized.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.