B-hGPRC5D MC38|BioMice百奥动物

Common name	B-hGPRC5D MC38	Catalog number	321822
Aliases	GPRC5D, G protein-coupled receptor class C group 5 member D	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Gprc5d gene was replaced by human GPRC5D coding sequence in B-hGPRC5D MC38 cells. Human GPRC5D is highly expressed on the surface of B-hGPRC5D MC38 cells.

Application

B-hGPRC5D MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hGPRC5D MC38 cells. The exogenous promoter and human GPRC5D coding sequence was inserted to replace part of murine exon 2 and all of exons 3 and 4. The insertion disrupts the endogenous murine Gprc5d gene, resulting in a non-functional transcript.

Protein expression analysis

GPRC5D expression analysis in B-hGPRC5D MC38 cells by flow cytometry.

Single cell suspensions from wild-type MC38 and B-hGPRC5D MC38 cultures were stained with species-specific anti-GPRC5D antibody. Human GPRC5D was detected on the surface of B-hGPRC5D MC38 cells but not wild-type MC38 cells. The 3-D10 clone of B-hGPRC5D MC38 cells was used for in vivo tumor growth assays.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hGPRC5D MC38 cells.

B-hGPRC5D MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hGPRC5D MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hGPRC5D MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6). At the end of the experiment, tumor cells were harvested and assessed for human GPRC5D expression by flow cytometry. As shown, human GPRC5D was highly expressed on the surface of tumor cells. Therefore, B-hGPRC5D MC38 cells can be used for in vivo efficacy studies of GPRC5D therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hGPRC5D MC38 cells. B-hGPRC5D MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hGPRC5D MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.