B-hFOLR1 MC38|BioMice百奥动物

Common name	B-hFOLR1 MC38	Catalog number	322242
Aliases	FBP, FOLR, NCFTD, Fralpha	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The human FOLR1 coding sequence was inserted into the exon 4 of mouse Folr1 in B-hFOLR1 MC38 cells. Human FOLR1 is highly expressed on the surface of B-hFOLR1 MC38 cells.

Application

B-hFOLR1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hFOLR1 MC38 cells. The human FOLR1 coding sequence was inserted into the exon 4 of mouse Folr1 in B-hFOLR1 MC38 cells. The insertion disrupts the endogenous murine Folr1 gene, resulting in a non-functional transcript.

Protein expression analysis

FOLR1 expression analysis in B-hFOLR1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hFOLR1 MC38 cultures were stained with species-specific anti-FOLR1 antibody. Human FOLR1 was detected on the surface of B-hFOLR1 MC38 cells but not wild-type MC38 cells. The 1-F01 clone of B-hFOLR1 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hFOLR1 MC38 cells. B-hFOLR1 MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=8 or 6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hFOLR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hFOLR1 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6), and on 31 days post inoculation, tumor cells were harvested and assessed for human FOLR1 expression by flow cytometry. As shown, human FOLR1 was highly expressed on the surface of tumor cells. Therefore, B-hFOLR1 MC38 cells can be used for in vivo efficacy studies of novel FOLR1 therapeutics.

Tumor growth curve & body weight changes

Subcutaneous homograft tumor growth of B-hFOLR1 MC38 cells. B-hFOLR1 MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into B-hFOLR1/hFOLR2 mice (female, 8-week-old, n=7). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hFOLR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor tissue

B-hFOLR1 MC38 cells were subcutaneously transplanted into B-hFOLR1/hFOLR2 mice (n=6), and on 31 days post inoculation, tumor cells were harvested and assessed for human FOLR1 expression by flow cytometry. As shown, human FOLR1 was highly expressed on the surface of tumor cells. Therefore, B-hFOLR1 MC38 cells can be used for in vivo efficacy studies of novel FOLR1 therapeutics.