B-hCD20 MC38|BioMice百奥动物

Common name	B-hCD20 MC38	Catalog number	310716
Aliases	MS4A1, Bp35, CVID5	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The human CD20 coding sequence was inserted into the ROSA26 locus in MC38 cells. Human CD20 is highly expressed on the surface of B-hCD20 MC38 cells.

Application

B-hCD20 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCD20 MC38 cells. The exogenous promoter and human CD20 coding sequence was inserted into the ROSA26 locus in murine.

Protein expression analysis

CD20 expression analysis in B-hCD20 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD20 MC38 cultures were stained with species-specific anti-CD20 antibody. Human CD20 was detected on the surface of B-hCD20 MC38 cells but not wild-type MC38 cells. The 2-A12 clone of B-hCD20 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCD20 MC38 cells. B-hCD20 MC38 cells and wild-type MC38 cells were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCD20 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

The subcutaneous tumor formation of B-hCD20 MC38 cells was slow, and the inoculation dose was recommended to be increased to 1x10⁷.