B-hCCR9 MC38|BioMice百奥动物

Common name	B-hCCR9 MC38	Catalog number	321825
Aliases	GPR28; CDw199; GPR-9-6; CC-CKR-9	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Ccr9 gene was replaced by human CCR9 coding sequence in B-hCCR9 MC38 cells. Human CCR9 is highly expressed on the surface of B-hCCR9 MC38 cells.

Application

B-hCCR9 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis

CCR9 expression analysis in B-hCCR9 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCCR9 MC38 cultures were stained with species-specific anti-CCR9 antibody. Human CCR9 was detected on the surface of B-hCCR9 MC38 cells but not wild-type MC38 cells. The 1-G09 clone of B-hCCR9 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCCR9 MC38 cells. B-hCCR9 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into heterozygous B-hCCR9 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCCR9 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tip:B-hCCR9 MC38 can not establish tumors in C57BL/6 mice, even in high dose (5E6 cells per mice).

Protein expression analysis of tumor cells

B-hCCR9 MC38 cells were subcutaneously transplanted into heterozygous B-hCCR9 mice (n=6), and on 49 days post inoculation, tumor cells were harvested and assessed for CCR9 expression by flow cytometry. As shown, human CCR9 was highly expressed on the surface of tumor cells. Therefore, B-hCCR9 MC38 cells can be used for in vivo efficacy studies of novel CCR9 therapeutics.