B-hCCL2 MC38|BioMice百奥动物

Common name	B-hCCL2 MC38	Catalog number	321866
Aliases	HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Ccl2 gene was replaced by human CCL2 coding sequence in B-hCCL2 MC38 cells. Human CCL2 can be secreted by B-hCCL2 MC38 cells.

Application

B-hCCL2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCCL2 MC38 cells. The exogenous promoter and human CCL2 coding sequence was inserted to replace part of murine exon 2~3. The insertion disrupts the endogenous murine Ccl2 gene, resulting in a non-functional transcript.

Protein expression analysis

CCL2 expression analysis in B-hCCL2 MC38 cells by ELISA. Human CCL2 was detected in the supernatant of B-hCCL2 MC38 cells but not wild-type MC38 cells. Mouse Ccl2 was not detected in the supernatant of wild-type MC38 cells. The 2-D07 clone of B-hCCL2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCCL2 MC38 cells. B-hCCL2 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCCL2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.