B-hANXA2 MC38|BioMice百奥动物

Common name	B-hANXA2 MC38	Catalog number	321942
Aliases	P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The mouse Anxa2 gene was replaced by human ANXA2 coding sequence in B-hANXA2 MC38 cells. Human ANXA2 is highly expressed on the surface of B-hANXA2 MC38 cells.

Application

B-hANXA2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hANXA2 MC38 cells. The exogenous promoter and human ANXA2 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Anxa2 gene, resulting in a non-functional transcript.

Protein expression analysis

ANXA2 expression analysis in B-hANXA2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hANXA2 MC38 cultures were stained with anti-ANXA2 antibody. Human ANXA2 was detected on the surface of B-hANXA2 MC38 cells and MC38 cells, as the antibody is crossly reactive with ANXA2 in human and mice. It has been verified that B-hANXA2 MC38 cells do not express mouse mRNA by RT-PCR. The 2-A01 clone of B-hANXA2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hANXA2 MC38 cells. B-hANXA2 MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diaANXA2er X short diaANXA2er². As shown in panel A, B-hANXA2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.