B-hADAM9 MC38|BioMice百奥动物

Common name	B-hADAM9 MC38	Catalog number	322015
Aliases	CORD9, MCMP, MDC9, Mltng	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The exogenous promoter and chimeric ADAM9 coding sequence containing human extracellular domain, mouse transmembrane domain and cytoplasmic domain was inserted to replace murine exon 5-6.

Application

B-hADAM9 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hADAM9 MC38 cells. The exogenous promoter and chimeric ADAM9 coding sequence containing human extracellular domain, mouse transmembrane domain and cytoplasmic domain were inserted to replace murine exons 5-6. The insertion disrupts the endogenous murine ADAM9 gene, resulting in a non-functional transcript.

Protein expression analysis

ADAM9 expression analysis in B-hADAM9 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hADAM9 MC38 cultures were stained with species-specific anti-ADAM9 antibody. Human ADAM9 was detected on the surface of B-hADAM9 MC38 cells but not wild-type MC38 cells. The 1-A05 clone of B-hADAM9 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hADAM9 MC38 cells. B-hADAM9 MC38 cells (3x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hADAM9 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.