B-Tg(hHER2/hTROP2) MC38|BioMice百奥动物

Common name	B-Tg(hHER2/hTROP2) MC38	Catalog number	321945
Aliases	ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2), erb-b2 receptor tyrosine kinase 2; TACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

模型验证

Description

The expression cassettes containing exogenous promoter and human HER2 chemic CDS and the expression cassettes containing exogenous promoter and human TROP2 CDS were randomly inserted into the genome of B-Tg(hHER2/hTROP2) MC38 cells. Human HER2 and TROP2 are highly expressed on the surface of B-Tg(hHER2/hTROP2) MC38 cells.

Application

B-Tg(hHER2/hTROP2) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis

hHER2/hTROP2 expression analysis in B-Tg(hHER2/hTROP2) MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(hHER2/hTROP2) MC38 cultures were stained with species-specific anti-HER2 and anti-TROP2 antibody. Human HER2 and TROP2 were detected on the surface of B-Tg(hHER2/hTROP2) MC38 cells but not wild-type MC38 cells. The MIX, 1-C07 and 1-D06 clone of B-Tg(hHER2/hTROP2) MC38 cells were used for in vivo tumor growth assays.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(hHER2/hTROP2) MC38 cells. B-Tg(hHER2/hTROP2) MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into heterozygous B-hHER2/hTROP2 mice (female, 27-week-old, n=10). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Tg(hHER2/hTROP2) MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Note that B-Tg(hHER2/hTROP2) MC38 cells successfully formed tumors only in heterozygous B-Tg(hHER2/hTROP2) mice.

Tumor growth curve of individual mice

B-Tg(hHER2/hTROP2) MC38 tumor cells growth of individual mice. B-Tg(hHER2/hTROP2) MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into heterozygous B-hHER2/hTROP2 mice (female, 27-week-old, n=10). As shown in panel, B-Tg(hHER2/hTROP2) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-Tg(hHER2/hTROP2) MC38 cells were subcutaneously transplanted into heterozygous B-hHER2/hTROP2 mice (female, 27-week-old, n=10), and on 42 days post inoculation, tumor cells were harvested and assessed for human TROP2 expression by flow cytometry. As shown, human TROP2 was highly expressed on the surface of tumor cells. Therefore, B-Tg(hHER2/hTROP2) MC38 cells can be used for in vivo efficacy studies of HER2/TROP2 therapeutics.

B-Tg(hHER2/hTROP2) MC38 cells were subcutaneously transplanted into heterozygous B-hHER2/hTROP2 mice (female, 27-week-old, n=10), and on 42 days post inoculation, tumor cells were harvested and assessed for human HER2 expression by flow cytometry. As shown, human HER2 was highly expressed on the surface of tumor cells. Therefore, B-Tg(hHER2/hTROP2) MC38 cells can be used for in vivo efficacy studies of HER2/TROP2 therapeutics.