B-Cd11b-EGFP-DTR-Luc mice
| Strain Name | C57BL/6JNifdc-Itgamtm1(EGFP-DTR-Luc)Bcgen/Bcgen | Common Name | B-Cd11b-EGFP-DTR-Luc mice |
| Background | C57BL/6JNifdc | Catalog number |
112825 |
|
Aliases |
NA | ||
模型验证
Description
- B-Cd11b-EGFP-DTR-Luc mice have the mouse Itgam (Cd11b) promoter to determine the expression of DTR (Diphtheria toxin receptor), EGFP (enhanced green fluorescent protein) and luciferase.
- Neutrophils, monocytes, macrophages, a part of NK cells and DCs will be traced by EGFP or Luciferase reporting system in B-Cd11b-EGFP-DTR-Luc mice.
- CD11b+ cells will be depleted in B-Cd11b-EGFP-DTR-Luc mice injected with DT (diphtheria toxin).
- The mice can be used to study the specific role of CD11b+ cells in immune response.
Gene targeting strategy for B-Cd11b-EGFP-DTR-Luc mice. CD11b co-express EGFP, the DTR for depletion of CD11b+ cells, and the luciferase for in vivo bioluminescent imaging (BLI) of CD11b+ cells. EGFP-DTR-Luc expression is evident in CD11b+ cells.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. Values are expressed as mean ± SEM.
Frequencies of CD11b+ EGFP+ cells and EGFP+ cells in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Cd11b-EGFP-DTR-Luc mice (Mut/+) (n=3, 9-week-old) injected with PBS or DT (50 ng/g body weight) for three consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of CD11b+ EGFP+ cells in total CD45+ cells. The frequencies of CD11b+ EGFP+ cells were decreased in heterozygous mice injected with DT.
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