Strain Name |
SD-CAGtm1(icre)Bcgen/Bcgen |
Stock No. |
211350 |
Common name |
B-CAG-iCre rats |
Gene symbol and name |
CAG promoter (a combination of artificially constructed promoter, which is composed of the early enhancer element of cytomegalovirus (CMV) and chicken beta-actin promoter) |
Strain of origin |
SD |
Chromosome | 4 |
Coat color |
White |
Dietary information |
Growth and reproduction diet for experimental mice |
Phenotype Analysis
B-CAG-iCre rats were bred to B-CAG-tdTomato cKI rats, tdTomato were detected in the double-positive rats.
Immunofluorescent images of tdTomato and iCre in the main organs of double-positive rats. Arrows indicate the expression of iCre. Scale bar, 25 µm.
Instructions of genomic DNA extraction
Numbering rules of toe clipping for identification of mouse/rat model
chematic representation of the toe clipping.
1. Kobayashi T, Kato-Itoh M, Yamaguchi T, Tamura C, Sanbo M, Hirabayashi M, Nakauchi H. Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato. Stem Cells Dev. 2012 Nov 1;21(16):2981-6. [PubMed: 22564063]
2. Ma Y, Yu L, Pan S, Gao S, Chen W, Zhang X, Dong W, Li J, Zhou R, Huang L, Han Y, Bai L, Zhang L, Zhang L. CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre-loxP-mediated lineage tracing. FEBS J. 2017 Oct;284(19):3262-3277. Epub 2017 Sep 19. [PubMed: 28763160]
3. Zhang H, Zheng Q, Chen-Tsai RY. Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases. Transgenic Res. 2021 Feb;30(1):91-104. [PubMed: 33481207]