Application

This CAG-tdTomato model is an efficient tool to study the tissue specification of Cre mice, when cross with the Cre mice.

Expression

Expressed gene

tdTomato, Red fluresence protein, report gene

Site of expression

CAG promotor, Extensive expression throughout the body

Gene editing strategy

A CAG-LSL-tdTomato-WPRE-pA cassette was placed between the exon 1 and exon 2 using C57BL/6 embryonic stem cells. This strain was maintained on a C57BL/6 genetic background.

Phenotype Analysis

Experimental design : T cells were isolated from peripheral blood in the single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

Experimental design : T cells were isolated from spleen in single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

Results: tdTomato+ cells were detected in the double-positive mice.

1. Chaushu S; Wilensky A; Gur C; Shapira L; Elboim M; Halftek G; Polak D; Achdout H; Bachrach G; Mandelboim O. 2012. Direct recognition of Fusobacterium nucleatum by the NK cell natural cytotoxicity receptor NKp46 aggravates periodontal disease. PLoS Pathog 8(3):e1002601. [PubMed:22457623]

2. Elboim M; Gazit R; Gur C; Ghadially H; Betser-Cohen G; Mandelboim O. 2010. Tumor immunoediting by NKp46. J Immunol 184(10):5637-44. [PubMed:20404273]

3. Ghadially H; Horani A; Glasner A; Elboim M; Gazit R; Shoseyov D; Mandelboim O. 2013. NKp46 regulates allergic responses. Eur J Immunol 43(11):3006-16. [PubMed: 23878025] 

4. Ghadially H; Ohana M; Elboim M; Gazit R; Gur C; Nagler A; Mandelboim O. 2014. NK Cell Receptor NKp46 Regulates Graft-versus-Host Disease. Cell Rep 7(6):1809-14. [PubMed: 24882008]

5. Glasner A; Ghadially H; Gur C; Stanietsky N; Tsukerman P; Enk J; Mandelboim O. 2012. Recognition and Prevention of Tumor Metastasis by the NK Receptor NKp46/NCR1. J Immunol 188(6):2509-15. [PubMed: 22308311]

VEGF signaling governs the initiation of biliary-mediated liver regeneration through the PI3K-mTORC1 axis.pdf

BNST GABAergic neurons modulate wakefulness over sleep and anesthesia.pdf

When using the B-CAG-tdTomato cKI  mouse strain in a publication, please indicate the originate from Biocytogen and stock #110148 in your Materials and Methods section.