Application

Expression

Expressed gene

iCre, improved cre recombinase, bacteriophage P1

Site of expression

The molecular is expressed on resting and activated NK cells

Gene editing strategy

An IRES-iCre sequence cassette was placed between the coding sequence of exon 7 and 3’UTR of the Nkp46 gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at least 300bp downstream of 3’UTR. This strain was maintained on a C57BL/6 genetic background.

Phenotype Analysis

NKp46 þ ILC3 cells from Washflox/flox and Washflox/floxNcr1-Cre mice were in situ hybridized with probes against Ahr promoter, followed by staining with antibodies against WASH and Arid1a (upper panel). White arrow head indicates Ahr promoters colocalized with Arid1a. Percentages of cells with Arid1a colocalized Ahr promoter were calculated (lower panel). At least 200 NKp46 þ ILC3 cells were counted. Scale bar, 5 mm.

(source: Xia P et al.,2017)

1. Xia P, Liu J, Wang S, Ye B, Du Y, Xiong Z, Han ZG, Tong L, Fan Z. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat Commun. 2017 Jun 7;8:15685. [PubMed: 28589939]

2. Wang S, Xia P, Huang G, Zhu P, Liu J, Ye B, Du Y, Fan Z. FoxO1-mediated autophagy is required for NK cell development and innate immunity. Nat Commun. 2016 Mar 24;7:11023. [PubMed: 27010363]

3. Elboim M; Gazit R; Gur C; Ghadially H; Betser-Cohen G; Mandelboim O. 2010. Tumor immunoediting by NKp46. J Immunol 184(10):5637-44. [PubMed:20404273]

4. Ghadially H; Horani A; Glasner A; Elboim M; Gazit R; Shoseyov D; Mandelboim O. 2013. NKp46 regulates allergic responses. Eur J Immunol 43(11):3006-16. [PubMed: 23878025] 

5. Ghadially H; Ohana M; Elboim M; Gazit R; Gur C; Nagler A; Mandelboim O. 2014. NK Cell Receptor NKp46 Regulates Graft-versus-Host Disease. Cell Rep 7(6):1809-14. [PubMed: 24882008]

6. Glasner A; Ghadially H; Gur C; Stanietsky N; Tsukerman P; Enk J; Mandelboim O. 2012. Recognition and Prevention of Tumor Metastasis by the NK Receptor NKp46/NCR1. J Immunol 188(6):2509-15. [PubMed: 22308311]

7. Gur C; Porgador A; Elboim M; Gazit R; Mizrahi S; Stern-Ginossar N; Achdout H; Ghadially H; Dor Y; Nir T; Doviner V; Hershkovitz O; Mendelson M; Naparstek Y; Mandelboim O. 2010. The activating receptor NKp46 is essential for the development of type 1 diabetes. Nat Immunol 11(2):121-8. [PubMed: 20023661]

STING signaling promotes NK cell antitumor immunity and maintains a reservoir of TCF-1+ NK cells.pdf

The ω-3 polyunsaturated fatty acid docosahexaenoic acid enhances NK-cell antitumor effector functions.pdf

When using the B-Nkp46-iCre mouse strain in a publication, please indicate the originate from Biocytogen and stock #110127 in your Materials and Methods section.