B-Rag2/Il2rg DKO mice
| Strain Name | C57BL/6N-Rag2tm1Bcgen Il2rgtm1Bcgen/Bcgen | Common Name | B-Rag2/Il2rg DKO mice |
| Background | C57BL/6N | Catalog number | 112937 |
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Aliases |
RAG-2; CD132, [g]c, gamma(c), gc, p64
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模型验证
The size and weight of thymus and spleen significantly reduced in B-Rag2/Il2rg DKO mice
Evans blue staining of lymph nodes
Frequency of leukocyte subpopulations in bone marrow
Frequency of leukocyte subpopulations in spleen
Frequency of leukocyte subpopulations in blood
The size and weight of thymus and spleen of B-Rag2/Il2rg DKO mice were significantly reduced compared to that of wild-type C57BL/6 mice. Thymuses and spleens were isolated from wild-type C57BL/6 mice and B-Rag2/Il2rg DKO mice (n=3, 7-week-old). A. Gross anatomy of thymuses; B. The size of spleen and thymus; C. Comparison of organ coefficients of spleen and thymus in wild-type C57BL/6 mice and B-Rag2/Il2rg DKO mice. Results showed that thymuses were not visualized in B-Rag2/Il2rg DKO mice. The size and weight of spleens in B-Rag2/Il2rg DKO mice were significantly reduced compared to that in wild-type C57BL/6 mice.
Lymph nodes were not visualized in B-Rag2/Il2rg DKO mice dyed by Evans blue. Wild-type C57BL/6 mice and B-Rag2/Il2rg DKO mice (n=3) were sacrificed and intravenously injected with 10% Evens blue in the planta pedis. The red arrows indicate the lymph nodes in inguinal, axillary and mesenteric site of the mice. No lymph node was visualized in B-Rag2/Il2rg DKO mice.
Frequency of leukocyte subpopulations in bone marrow by flow cytometry. Bone marrow cells were isolated from male wild-type C57BL/6 mice and homozygous B-Rag2/Il2rg DKO mice (n=3, 6-week-old). A. Flow cytometry analysis of the bone marrow cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. T cells, including CD4+ T cells, CD8+ T cells and Tregs, B cells and NK cells were not detectable in B-Rag2/Il2rg DKO mice. Frequency of neutrophils, monocytes and macrophages were increased in B-Rag2/Il2rg DKO mice compared to that in C57BL/6 mice, demonstrating that the development of T cells, B cells and NK cells in bone marrow were completely inhibited in B-Rag2/Il2rg DKO mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from male wild-type C57BL/6 mice and homozygous B-Rag2/Il2rg DKO mice (n=3, 6-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. T cells, including CD4+ T cells, CD8+ T cells and Tregs, B cells and NK cells were not detectable in B-Rag2/Il2rg DKO mice. Frequency of dendritic cells, neutrophils, monocytes and macrophages were increased in B-Rag2/Il2rg DKO mice compared to that in C57BL/6 mice, demonstrating that the development of T cells, B cells and NK cells in spleen were completely inhibited in B-Rag2/Il2rg DKO mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from male wild-type C57BL/6 mice and homozygous B-Rag2/Il2rg DKO mice (n=3, 6-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. T cells, including CD4+ T cells, CD8+ T cells and Tregs, B cells and NK cells were not detectable in B-Rag2/Il2rg DKO mice. Frequency of dendritic cells, neutrophils and macrophages were increased in B-Rag2/Il2rg DKO mice compared to that in C57BL/6 mice, demonstrating that the development of T cells, B cells and NK cells in blood were completely inhibited in B-Rag2/Il2rg DKO mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
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