B-hTTR*V30M mice
| Strain Name |
C57BL/6-Ttrtm1(TTR*V30M)Bcgen/Bcgen
|
Common Name | B-hTTR*V30M mice |
| Background | C57BL/6 | Catalog number | 112386 |
|
Aliases |
CTS, TTN, ATTR, CTS1, PALB, TBPA, HEL111, HsT2651 | ||
模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of TTR*V30M mRNA expression in wild-type C57BL/6 mice and B-hTTR*V30M mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and heterozygous B-hTTR*V30M mice (H/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TTR primers. Mouse Ttr mRNA was detectable in wild-type C57BL/6 mice and heterozygous B-hTTR*V30M mice. Human TTR mRNA was detectable only in heterozygous B-hTTR*V30M mice but not in wild-type mice.
Strain specific TTR expression analysis in homozygous B-hTTR*V30M mice by ELISA. Plasma were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTTR*V30M mice (H/H) and analyzed by ELISA with species-specific TTR ELISA kit. Mouse TTR was detectable in wild-type mice (A). Human TTR was exclusively detectable in homozygous B-hTTR*V30M mice (B).
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