B-hTSPAN2 mice
| Strain Name |
C57BL/6-Tspan2tm2(TSPAN2)Bcgen/Bcgen
|
Common Name | B-hTSPAN2 mice |
| Background | C57BL/6 | Catalog number |
112876 |
|
Related Genes |
NET3, TSN2, TSPAN-2 |
||
|
NCBI Gene ID |
10100 | ||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of TSPAN2 protein expression in homozygous B-hTSPAN2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTSPAN2 mice (H/H), and then analyzed by western blot with cross reactive anti-TSPAN2 antibody (Proteintech, 20463-1-AP). 40 μg total proteins were loaded for western blotting analysis. TSPAN2 was detected in heart, liver, lung, stomach, urinary bladder and ovary of both wild-type mice and homozygous mice.
Strain specific analysis of TSPAN2 mRNA expression in wild-type C57BL/6 mice and B-hTSPAN2 mice by RT-PCR. Lung and stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTSPAN2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TSPAN2 primers. Mouse Tspan2 mRNA was detectable only in wild-type C57BL/6 mice. Human TSPAN2 mRNA was detectable only in homozygous B-hTSPAN2 mice but not in wild-type mice.
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