B-hTRPM4 mice
| Strain Name |
C57BL/6N-Trpm4tm1(TRPM4)Bcgen/Bcgen
|
Common Name | B-hTRPM4 mice |
| Background | C57BL/6N | Catalog number |
112597 |
|
Related Genes |
EKVP6, LTrpC, PFHB1B, TRPM4B, hTRPM4 |
||
|
NCBI Gene ID |
54795 | ||
模型验证
Description
Protein expression analysis
- TRPM4 is a calcium-activated non selective cation channel which mediated cell membrane depolarization.
- Homozygous mice expressed chimeric TRPM4 and developed normally.。
- This model is useful in studying the role of TRPM4 in cadiomyocytes, neurons and cochlea hair cells.
mRNA expression analysis
Analysis of TRPM4 mRNA expression in wild-type C57BL/6N mice and B-hTRPM4 mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hTRPM4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Trpm4 and human TRPM4 primers. Mouse Trpm4 mRNA was only detectable in wild-type mice, human TRPM4 mRNA was exclusively detectable in homozygous B-hTRPM4 mice but not in wild-type mice.
Western blot analysis of TRPM4 protein expression in homozygous B-hTRPM4 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hTRPM4 mice (H/H), and then analyzed by western blot with anti-TRPM4 antibody (Abcam, ab106200). 40 μg total proteins were loaded for western blotting analysis. TRPM4 was detectable in brain, heart (at a low level), lung, liver, large intestine and stomach.
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