B-hTM4SF1 mice
| Strain Name |
C57BL/6N-Tm4sf1tm1(TM4SF1)Bcgen/Bcgen
|
Common Name | B-hTM4SF1 mice |
| Background | C57BL/6N | Catalog number | 111426 |
|
Aliases |
L6, H-L6, M3S1, TAAL6 | ||
模型验证
Description
Protein expression analysis
- Expression: TM4SF1 is a protein with four transmembrane domains, which is highly expressed on lung, breast, colon, and ovarian carcinomas. It is also present on normal endothelial cells.
- Application: This mice model can be used for pharmacodynamics and safety evaluation of colon, breast and other cancers.
mRNA expression analysis
Strain specific analysis of TM4SF1 mRNA expression in wild-type C57BL/6 mice and B-hTM4SF1 mice by RT-PCR. Lung RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hTM4SF1 mice (H/H), then cDNA libraries was synthesized by reverse transcription, followed by PCR with mouse or human TM4SF1 primers. Mouse Tm4sf1 mRNA was only detectable in wild-type mice. Human TM4SF1 mRNA was exclusively detectable in homozygous B-hTM4SF1 mice but not in wild-type mice.
Strain specific TM4SF1 expression analysis in homozygous B-hTM4SF1 mice by flow cytometry. Lung endothelial cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTM4SF1 mice (H/H), and analyzed by flow cytometry with anti-humanTM4SF1 antibody (R&D, FAB8164R). hTM4SF1 was detectable on lung endothelial cells of homozygous mice.
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