B-hTCIRG1 mice
| Strain Name |
C57BL/6-Tcirg1tm1(TCIRG1)Bcgen/Bcgen
|
Common Name | B-hTCIRG1 mice |
| Background | C57BL/6 | Catalog number |
112214 |
|
Aliases |
TCIRG1: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, OC-116kDa |
||
模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of TCIRG1 mRNA expression in wild-type C57BL/6 mice and B-hTCIRG1 mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTCIRG1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TCIRG1 primers. Mouse Tcirg1 mRNA was detectable only in wild-type C57BL/6 mice. Human TCIRG1 mRNA was detectable only in homozygous B-hTCIRG1 mice but not in wild-type mice.
Western blot analysis of TCIRG1 protein expression in homozygous B-hTCIRG1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTCIRG1 mice (H/H), and then analyzed by western blot with cross-reactive anti-TCIRG1 antibody. 20 μg total proteins were loaded for western blotting analysis. TCIRG1 was detected in spleen, lymph node, lung, adrenal gland and bone marrow.
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