B-hSLC4A1 mice
| Strain Name |
C57BL/6N-Slc4a1tm1(SLC4A1)Bcgen/Bcgen
|
Common Name | B-hSLC4A1 mice |
| Background | C57BL/6N | Catalog number |
112942 |
|
Related Genes |
AE1, BND3, CD233, CHC, DI, EMPB3, EPB3, FR, RTA1A, SAO, SPH4, SW, WD, WD1, WR |
||
|
NCBI Gene ID |
6521 | ||
模型验证
Description
mRNA expression analysis
The SLC4A1 (Band 3) protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. SLC4A1 is a 90-100 kDa protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of SLC4A1, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin.
Protein expression analysis
Strain specific hCD233/SLC4A1 expression analysis in homozygous B-hSLC4A1 mice(H/H) by flow cytometry. Blood were collected from wild-type C57BL/6N mice(+/+) and B-hSLC4A1 mice(H/H) , and analyzed by flow cytometry with anti-human CD233/SLC4A1 antibody (Miltenyi biotec, 130-117-825). Human CD233/SLC4A1 was detectable in homozygous B-hSLC4A1 mice.
Strain specific analysis of SLC4A1 mRNA expression in wild-type C57BL/6N mice and B-hSLC4A1 mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hSLC4A1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SLC4A1 primers. Mouse Slc4a1 mRNA was detectable in wild-type mice. Human SLC4A1 mRNA was detectable only in homozygous B-hSLC4A1 mice(H/H) but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技