B-hSEMA7A mice
| Strain Name | C57BL/6JNifdc-Sema7atm1(SEMA7A)Bcgen/Bcgen | Common Name | B-hSEMA7A mice |
| Background | C57BL/6JNifdc | Catalog number | 112517 |
|
Aliases |
SEMA7A: JMH, CD108, SEMAL, CDw108, PFIC11, SEMAK1, H-Sema-L, H-SEMA-K1 | ||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of SEMA7A protein expression in homozygous B-hSEMA7A mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA7A mice (H/H), and then analyzed by western blot with species-specific anti-SEMA7A antibody. 40 μg total proteins were loaded for western blotting analysis. SEMA7A was detected in brain, testis, lung and colon.
Strain specific analysis of SEMA7A mRNA expression in wild-type C57BL/6 mice and B-hSEMA7A mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA7A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SEMA7A primers. Mouse Sema7a mRNA was detectable only in wild-type C57BL/6 mice. Human SEMA7A mRNA was detectable only in homozygous B-hSEMA7A mice but not in wild-type mice.
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