Strain specific analysis of ROR1 gene expression in wild-type C57BL/6 mice and B-hROR1 mice by RT-PCR. ROR1 mRNA was detectable in lung of wild-type C57BL/6 mice and homozygous B-hROR1 mice (H/H) due to the high genetic similarity, and we sequenced and confirmed that the extracellular region of homozygous B-hROR1 mice (H/H) is the human ROR1 sequence.
Protein expression analysis
Strain specific ROR1 expression analysis in homozygous B-hROR1 mice by western blot. Tissues were collected from WT mice (+/+) and homozygous B-hROR1 (H/H) mice, and analyzed by western blot with anti-ROR1 antibody. ROR1 was detectable in embryo of WT mice (+/+) and homozygous B-hROR1 mice (H/H) due to the cross-reactivity of antibodies.