Strain Name |
C57BL/6N-Pscatm1(PSCA)Bcgen/Bcgen
|
Common Name |
B-hPSCA mice |
Background | C57BL/6N | Catalog number |
110991 |
Related Genes |
PRO232; lncPSCA |
mRNA expression analysis
Strain specific analysis of PSCA gene expression in wild-type mice and B-hPSCA mice by RT-PCR. Mouse Psca mRNA was detectable only in stomach of wild-type mice (+/+). Human PSCA mRNA was detectable only in homozygous B-hPSCA mice (H/H) but not in wild-type C57BL/6 mice.
PSCA protein expression analysis in wild-type mice and B-hPSCA mice by western blotting. PSCA was both detectable in B-hPSCA mice and wild-type mice because the antibody has cross reaction between human and mouse. But, it has been confirmed the PSCA sequence was humanization successfully.