B-hPROK2 mice
| Strain Name | C57BL/6JNifdc-Prok2tm1(PROK2)Bcgen/Bcgen | Common Name | B-hPROK2 mice |
| Background | C57BL/6JNifdc | Catalog number |
112514 |
|
Aliases |
PROK2:BV8; HH4; PK2; KAL4; MIT1 | ||
模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of PROK2 mRNA expression in wild-type C57BL/6 mice and B-hPROK2 mice by RT-PCR. Testis RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hPROK2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human PROK2 primers. Mouse PROK2 mRNA was only detectable in wild-type C57BL/6 mice. Human PROK2 mRNA was only detectable in homozygous B-hPROK2 mice but not in wild-type mice.
Western blot analysis of PROK2 protein expression in homozygous B-hPROK2 mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hPROK2 mice (H/H), and then analyzed by western blot with anti-PROK2 antibody. 40 μg total proteins were loaded for western blotting analysis. PROK2 was detected in hippocampus, cerebellum, bladder and spleen.
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