B-hMUC1 mice
| Strain Name | C57BL/6-Muc1tm1(MUC1)Bcgen/Bcgen | Common Name | B-hMUC1 mice |
| Background | C57BL/6 | Catalog number | 110867 |
|
Aliases |
MUC1 (ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM) | ||
模型验证
mRNA expression analysis
Protein expression analysis
B-hMUC1 MC38 can't grow on wild-type mice
B-hMUC1 MC38 can grow on B-hMUC1 mice
Strain specific analysis of MUC1 gene expression in wild-type C57BL/6 mice and hMUC1 mice by RT-PCR. Mouse Muc1 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice (+/+). Human MUC1 mRNA was detectable only in homozygous B-hMUC1 mice (H/H), but not in wild-type mice.
Western blot analysis of MUC1 protein expression in homozygous B-hMUC1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMUC1 mice(H/H), and then analyzed by western blot with anti-MUC1 antibody. 40 μg total proteins were loaded for western blotting analysis. Human and mouse MUC1 was detected in wild-type mice and homozygous B-hMUC1 mice due to the cross-reactivity of antibodies.
Subcutaneous homograft tumor growth of B-hMUC1 MC38 cells. B-hMUC1 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into wild-type C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC1 MC38 cells were unable to establish tumors in wild-type C57BL/6 mice.
Subcutaneous homograft tumor growth of B-hMUC1 MC38 cells. B-hMUC1 MC38 cells(2-C07 clone) (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hMUC1 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
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