Strain Name |
C57BL/6N-Miftm1(MIF)Bcgen Ddttm1(DDT)Bcgen/Bcgen
|
Common Name | B-hMIF/hDDT mice |
Background | C57BL/6N | Catalog number |
121219 |
Related Genes |
GIF, GLIF, MMIF; D-DT, DDCT, MIF-2, MIF2 |
||
NCBI Gene ID |
4282; 1652 |
Strain specific MIF expression analysis in homozygous B-hMIF/hDDT mice by ELISA. Serum were collected from wild-type C57BL/6N mice (+/+) (n=2, famle, 7w) and homozygous B-hMIF/hDDT mice(H/H;H/H) (n=3, famle, 7w) stimulated with LPS in vivo (20 μg/mice, stimulation for 4 hours, i.p.), and analyzed by ELISA with species-specific MIF ELISA kit. Expression level of mouse and human MIF were analyzed by ELISA (mouse MIF ELISA: abcam, ab209885; human MIF ELISA: RD, DMF00B). Mouse MIF was detectable in wild-type mice. Human MIF was exclusively detectable in homozygous B-hMIF mice but not in wild-type mice.
Western blot analysis of DDT protein expression in homozygous B-hMIF/hDDT mice. Various tissue lysates were collected from homozygous B-hMIF/hDDT mice (H/H;H/H), and then analyzed by western blot with anti-DDT antibody(Abcam, ab115785). 40 μg total proteins were loaded for western blotting analysis. DDT was detected in liver and testis. (HO: homozygous B-hMIF/hDDT mice (H/H;H/H); +/+: wild-type C57BL/6N mice (+/+)).