B-hMELTF mice
| Strain Name | C57BL/6-Meltftm1(MELTF)Bcgen/Bcgen | Common Name | B-hMELTF mice |
| Background | C57BL/6 | Catalog number |
111964 |
|
Aliases |
MTf; MFI2; MTF1; CD228; MAP97 |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of MELTF protein expression in homozygous B-hMELTF mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hMELTF mice (H/H), and then analyzed by western blot with anti-MELTF antibody. 40 μg total proteins were loaded for western blotting analysis. MELTF was detected in testis of wildtype mice (+/+) and homozygous B-hMELTF mice (H/H), as the antibody is crossly reactive with human and mice.
Strain specific analysis of MELTF mRNA expression in wild-type C57BL/6 mice and B-hMELTF mice by RT-PCR. Brain and kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hMELTF mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MELTF primers. Mouse Meltf mRNA was detectable only in wild-type C57BL/6 mice. Human MELTF mRNA was detectable only in homozygous B-hMELTF mice but not in wild-type mice.
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