B-hMDR1 mice
| Strain Name |
C57BL/6-Mdr1tm1(MDR1)Bcgen/Bcgen
|
Common Name | B-hMDR1 mice |
| Background | C57BL/6 | Catalog number |
112684 |
|
Aliases |
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170 |
||
模型验证
mRNA expression analysis
Protein expression analysis
MDR1 mRNA expression analysis in wild-type C57BL/6 mice and B-hMDR1 mice by RT-PCR. Liver RNA was isolated from wild-type C57BL/6 mice (+/+) and B-hMDR1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Mdr1a, Mdr1b primers and human MDR1 primers. Human MDR1 mRNA was only detectable in B-hMDR1 mice. Mouse Mdr1a and Mdr1b mRNA were only detectable in wild type mice but not in B-hMDR1 mice.
Western blot analysis of MDR1 protein expression in homozygous B-hMDR1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMDR1 mice (H/H), and then analyzed by western blot with anti-MDR1 antibody. 40μg total proteins were loaded for western blotting analysis. MDR1 was detectable in lung, spleen, heart, kidney and brain of wild-type mice and B-hMDR1 mice. Because the antibody used in the experiment has species-cross recognition. Knock-out mice tissue as a negative control. Refer to the mRNA results, the protein in B-hMDR1 mice was expressed by human MDR1 mRNA sequences.
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