B-hLOX1 mice
| Strain Name | C57BL/6N-Olr1tm2(OLR1)Bcgen/Bcgen | Common Name | B-hLOX1 mice |
| Background | C57BL/6N | Catalog number |
111978 |
|
Aliases |
LOX1, LOXIN, SLOX1, CLEC8A, SCARE1 | ||
模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of LOX1 mRNA expression in wild-type C57BL/6 mice and B-hLOX1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LOX1 primers. Mouse Lox1 mRNA was detectable only in wild-type C57BL/6 mice. Human LOX1 mRNA was detectable only in homozygous B-hLOX1 mice but not in wild-type mice. And the PCR products were confirmed with Sanger sequencing.
Strain specific LOX1 expression analysis in homozygous B-hLOX1 mice by flow cytometry. Bone marrow-derived dendritic cells(BMDC) were cultured from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H) stimulated with LPS (1μg/mL , stimulation for 18 hour), and analyzed by flow cytometry with species-specific anti-LOX1 antibody. Mouse LOX1 was detectable on BMDC of wild-type mice. Human LOX1 was detectable on BMDC of homozygous B-hLOX1 mice.
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