B-hLAT1 mice
| Strain Name |
C57BL/6-Slc7a5tm1(SLC7A5)Bcgen/Bcgen
|
Common Name | B-hLAT1 mice |
| Background | C57BL/6 | Catalog number | 110998 |
| Aliases |
SLC7A5, 4F2LC, CD98, D16S469E, E16, LAT1, MPE16, solute carrier family 7 member 5 [Homo sapiens |
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模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of LAT1 gene expression in wild type (WT) mice and B-hLAT1 mice by RT-PCR. Mouse Slc7a5 mRNA was detectable only in brain of WT mice (+/+). Human SLC7A5 mRNA was detectable only in homozygous B-hLAT1 mice (H/H) but not in WT mice (+/+).
Strain specific LAT1 expression analysis in homozygous B-hLAT1 mice by western blot. Brain was collected from wild type (WT) mice (+/+) and homozygous B-hLAT1 mice (H/H), and analyzed by western blot with anti-LAT1 antibody. LAT1 was detectable in WT mice (+/+) and homozygous B-hLAT1 mice (H/H) due to the cross-reactivity of antibodies. Human LAT1 was exclusively detectable in homozygous B-hLAT1 mice (H/H).
Protein expression analysis in brain
Strain specific LAT1 expression analysis in homozygous B-hLAT1 mice by Immunofluorescence staining. Brain was collected from wild type (WT) mice and homozygous B-hLAT1 mice (female,8 week old) and processed into paraffin sections. The paraffin sections were stained using an species-specific anti-human LAT1 antibody (green). Brain tissues were co-stained with an anti-mouse CD31 antibody to visualize microvascular endothelial cells (red). Representative results of WT mice (A) and homozygous B-hLAT1 mice (B) are shown. The results indicated that human LAT1 was exclusively detectable on brain microvascular endothelium of homozygous B-hLAT1 mice, but not in WT mice.
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