B-hIBSP mice
| Strain Name | C57BL/6N-Ibsptm1(IBSP)Bcgen/Bcgen | Common Name | B-hIBSP mice |
| Background | C57BL/6N | Catalog number |
110930 |
|
Aliases |
IBSP: BSP, BNSP, SP-II, BSP-II |
||
模型验证
Protein expression analysis
mRNA expression analysis
Strain specific IBSP expression analysis in homozygous B-hIBSP mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H) and analyzed by ELISA with IBSP ELISA kit. Mouse IBSP was detectable in wild-type mice and homozygous B-hIBSP mice as the kit has partly cross reactions with human and mouse (A). Human IBSP was exclusively detectable in homozygous B-hIBSP mice but not in wild-type mice (B).
Strain specific analysis of IBSP mRNA expression in wild-type C57BL/6 mice and B-hIBSP mice by RT-PCR. Bone marrow RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IBSP primers. Mouse Ibsp mRNA was detectable only in wild-type C57BL/6 mice. Human IBSP mRNA was detectable only in homozygous B-hIBSP mice but not in wild-type mice.
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