B-hHMMR mice
| Strain Name | C57BL/6-Hmmrtm1(HMMR)Bcgen/Bcgen | Common Name | B-hHMMR mice |
| Background | C57BL/6 | Catalog number |
111561 |
|
Aliases |
HMMR: CD168, IHABP, RHAMM |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of HMMR protein expression in homozygous B-hHMMR mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hHMMR mice (H/H), and then analyzed by western blot with species-specific anti-HMMR antibody. 30 μg total proteins were loaded for western blotting analysis. HMMR was detected in thymus.
Strain specific analysis of HMMR mRNA expression in wild-type C57BL/6 mice and B-hHMMR mice by RT-PCR. Thymus RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hHMMR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human HMMR primers. Mouse Hmmr mRNA was detectable only in wild-type C57BL/6 mice. Human HMMR mRNA was detectable only in homozygous B-hHMMR mice but not in wild-type mice.
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