B-hFSHB mice
| Strain Name | C57BL/6N-Fshbtm1(FSHB)Bcgen/Bcgen | Common Name | B-hFSHB mice |
| Background | C57BL/6N | Catalog number |
111475 |
|
Aliases |
HH24 |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of FSHB protein expression in homozygous B-hFSHB mice. Various tissue lysates were collected from C57BL/6 wild-type mice (+/+) and homozygous B-hFSHB mice (H/H), and then analyzed by western blot with anti-FSHB antibody. 30 μg total proteins were loaded for western blotting analysis. Human FSHB was detected in pituitary gland of homozygous B-hFSHB mice.
Strain specific analysis of FSHB mRNA expression in wild-type C57BL/6 mice and homozygous B-hFSHB mice by RT-PCR. Pituitary gland RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hFSHB mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FSHB primers. Mouse Fshb mRNA was detectable only in wild-type C57BL/6 mice. Human FSHB mRNA was detectable only in homozygous B-hFSHB mice but not in wild-type mice.
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