B-hEVA-1 mice
| Strain Name | C57BL/6N-Eva1tm1(EVA-1)Bcgen/Bcgen | Common Name | B-hEVA-1 mice |
| Background | C57BL/6N | Catalog number | 112351 |
|
Aliases |
EVA-1(DFNB111, EVA, EVA1) | ||
模型验证
mRNA and protein expression analysis
Protein expression analysis in thymus
Strain specific analysis of EVA-1 gene expression in wild-type mice and B-hEVA-1 mice by RT-PCR. Mouse Eva1 mRNA was detectable in thymus of wild-type mice (+/+). Human EVA-1 mRNA was detectable only in homozygous B-hEVA-1 mice(H/H) but not in wild-type mice.
Strain specific EVA-1 expression analysis in homozygous B-hEVA-1 mice by flow cytometry. Granulocytes, macrophages, dendritic cells from blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEVA-1 mice(H/H), and analyzed by flow cytometry with anti-EVA-1 antibody. Human EVA-1 was detectable in wild-type mice and homozygous B-hEVA-1 mice, as the antibody is crossly reactive with EVA-1 in human and mice.
Strain specific EVA-1 expression analysis in homozygous B-hEVA-1 mice by flow cytometry. DN3 cells, epithelial cells from thymus were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEVA-1 mice(H/H), and analyzed by flow cytometry with anti-EVA-1 antibody. EVA-1 was detectable only in wild-type mice but not in B-hEVA-1 mice .
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