B-hEREG mice
| Strain Name | C57BL/6N-Eregtm1(EREG)Bcgen/Bcgen | Common Name | B-hEREG mice |
| Background | C57BL/6N | Catalog number |
111476 |
|
Aliases |
ER; Ep; EPR |
||
模型验证
mRNA expression analysis
Protein expression analysis
Strain specific analysis of EREG mRNA expression in wild-type C57BL/6 mice and B-hEREG mice by RT-PCR. Small intestine RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEREG mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EREG primers. Mouse Ereg mRNA was detectable only in wild-type C57BL/6 mice. Human EREG mRNA was detectable only in homozygous B-hEREG mice but not in wild-type mice.
Strain specific EREG expression analysis in homozygous B-hEREG mice by ELISA. Colon homogenate were collected from wild-type mice C57BL/6 mice (+/+) and homozygous B-hEREG mice (H/H), and analyzed by ELISA with species-specific EREG ELISA kit. Human EREG was exclusively detectable in homozygous B-hEREG mice but not in wild-type mice (Each mg of total protein contains approximately 150 pg of EREG).
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