B-hEGFL7 mice
| Strain Name | C57BL/6-Egfl7tm1(EGFL7)Bcgen/Bcgen | Common Name | B-hEGFL7 mice |
| Background | C57BL/6 | Catalog number |
111238 |
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Aliases |
EGFL7: NEU1, VE-STATIN, ZNEU1 |
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模型验证
Protein expression analysis
mRNA expression analysis
Strain specific EGFL7 expression analysis in homozygous B-hEGFL7 mice by ELISA. Serum and lung were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEGFL7 mice (H/H), and then analyzed by ELISA with species-specific EGFL7 ELISA kit. Human EGFL7 was exclusively detectable in serum (A) and lung (B) in homozygous B-hEGFL7 mice but not in wild-type mice.
Strain specific analysis of EGFL7 mRNA expression in wild-type C57BL/6 mice and B-hEGFL7 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEGFL7 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EGFL7 primers. Mouse Egfl7 mRNA was detectable only in wild-type C57BL/6 mice. Human EGFL7 mRNA was detectable only in homozygous B-hEGFL7 mice but not in wild-type mice.
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