B-hCDH1 mice
| Strain Name |
C57BL/6N-Cdh1tm1(CDH1)Bcgen/Bcgen
|
Common Name | B-hCDH1 mice |
| Background | C57BL/6N | Catalog number |
111100 |
|
Aliases |
CDH1: UVO, CDHE, ECAD, LCAM, Arc-1, BCDS1, CD324 | ||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of CDH1 protein expression in homozygous B-hCDH1 mice. Colon tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hCDH1 mice (H/H), and then analyzed by western blot with species-specific anti-CDH1 antibody. 40 μg total proteins were loaded for western blotting analysis. Human CDH1 was detectable in colon of homozygous B-hCDH1 mice.
Strain specific analysis of CDH1 mRNA expression in wild-type C57BL/6 mice and B-hCDH1 mice by RT-PCR. Colon RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCDH1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CDH1 primers. Mouse Cdh1 mRNA was detectable only in wild-type C57BL/6 mice. Human CDH1 mRNA was detectable only in homozygous B-hCDH1 mice but not in wild-type mice.
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