mRNA expression analysis

Strain specific analysis of CD38 gene expression in WT and hCD38 mice by RT-PCR. Mouse Cd38 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CD38 mRNA was detectable only in H/H, but not in +/+ mice. 

Strain specific analysis of CD38 gene expression in wild-type C57BL/6 mice and B-hCD38 mice by RT-qPCR. The mRNA expression of CD38 in homozygous B-hCD38 mice (H/H) was similar to those in the wild-type C57BL/6 mice (+/+), demonstrating that introduction of hCD38 in place of its mouse counterpart does not change the CD38 expression. Values are expressed as mean ± SEM. Significance was determined by unpaired t-test.  *P < 0.05, **P < 0.01, ***P < 0.001. 

Strain specific CD38 expression analysis in homozygous B-hCD38 mice by flow cytometry. Blood were collected from WT and homozygous B-hCD38 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CD38 antibody. Mouse CD38 was detectable in WT mice. Human CD38 was exclusively detectable in homozygous B-hCD38 but not WT mice.

Combination of anti-human CD38 antibody with the immune cells in B-hCD38 mice

Panel 1: mTER-119, G-anti-hIgG-AF647
Panel 2: Live/Dead, mCD45, mCD3, mCD4, mCD8a, mNK1.1, mFoxp3, G-anti-hIgG-AF647
Panel 3: Live/Dead, mCD45, mCD11b, mCD14, mF4/80, G-anti-hIgG-AF647
Panel 4: Live/Dead, mCD45, mCD3, mCD4, mCD8a, mNK1.1, mFoxp3, hCD38-APC
Panel 5: Live/Dead, mCD45, mCD11b, mCD14, mF4/80, hCD38-APC

Anti-tumor activity of anti-human CD38 antibody in B-hCD38 mice. (A) Anti-human CD38 antibody (in house) inhibit B-hCD38-luc E.G7-OVA tumor growth in B-hCD38 mice. Murine T lymphocytoma B-hCD38-luc E.G7-OVA cells were injected by tail vein into homozygous B-hCD38 mice (female, 6 week-old, n=6). Mice were grouped when total flux reached approximately 10⁶ Ig, at which time they were treated with anti-human CD38 antibodies indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human CD38 antibodies were efficacious in controlling tumor growth. Values are expressed as mean ± SEM.

In vivo luciferase images of B-hCD38-Luc E.G7-OVA cells. Mice were grouped when total flux reached approximately 106 Ig, at which time they were treated with anti-human CD38 antibodies. Signal intensity and body weight were measured twice a week. Imaging was performed on days 0, 3, 7 and 10. Values are expressed as mean ± SEM.