B-hAMIGO2 mice
| Strain Name | C57BL/6N-Amigo2tm1(AMIGO2)Bcgen/Bcgen | Common Name | B-hAMIGO2 mice |
| Background | C57BL/6N | Catalog number | 111658 |
|
Aliases |
AMIGO2: ALI1, AMIGO-2, DEGA | ||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of AMIGO2 protein expression in homozygous B-hAMIGO2 mice. Various tissue lysates were collected from C57BL/6 wildtype (+/+) mice and homozygous B-hAMIGO2 (H/H) mice, and then analyzed by western blot with anti-AMIGO2 antibody. 40 μg total proteins were loaded for western blotting analysis. AMIGO2 was detected in lung, kidney, stomach and spleen.
Strain specific analysis of AMIGO2 mRNA expression in wild-type C57BL/6 mice and B-hAMIGO2 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hAMIGO2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human AMIGO2 primers. Mouse Amigo2 mRNA was detectable only in wild-type C57BL/6 mice. Human AMIGO2 mRNA was detectable only in homozygous B-hAMIGO2 mice but not in wild-type mice.
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