B-hTNFR2 mice(C)
| Strain Name | BALB/c-Tnfrsf1btm1(TNFRSF1B)Bcgen/Bcgen | Common Name | B-hTNFR2 mice(C) |
| Background | BALB/cCrSlcNifdc | Catalog number | 112644 |
|
Aliases |
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b) | ||
模型验证
Protein expression analysis in T cells
Protein expression analysis in Tregs
Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).
Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).
In vivo efficacy of anti-human TNFR2 antibody
Antitumor activity of anti-human TNFR2 antibody in B-hTNFR2 mice(C). (A) Anti-human TNFR2 antibody inhibited CT26.WT tumor growth in B-hTNFR2 mice(C). Murine colon cancer CT26.WT cells were subcutaneously implanted into homozygous B-hTNFR2 mice(C) (female, 7-week-old, n=6). Mice were grouped when tumor volume reached approximately 40-60 mm3, at which time they were intraperitoneally injected with anti-human TNFR2 antibody indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies. Values are expressed as mean ± SEM. (The hTNFR2 Ab1 was provided by the client)
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技