B-hSIRPA/hCD47/hCD3E/hCD28 mice
| Strain Name |
C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Cd3etm2(CD3E)Bcgen Cd28tm1(CD28)Bcgen/Bcgen
|
Common Name | B-hSIRPA/hCD47/hCD3E/hCD28 mice |
| Background | C57BL/6 | Catalog number | 111874 |
|
Related Genes |
PD-1 (Programmed death-1) ； SIRPA, BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, signal regulatory protein alpha; CD47, IAP, MER6, OA3, CD47 molecule; CD3E, IMD18, T3E, TCRE, CD3e molecule; CD28, Tp44, CD28 molecule |
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模型验证
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD28 expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice (+/+). Human CD28 was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific SIRPA expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-SIRPA antibody. Mouse SIRPA was detectable in WT mice and B-hSIRPA/hCD47/hCD3E/hCD28 mice due to the cross-reactivity of antibodies. Human SIRPA was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD47 expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD47 antibody. Mouse CD47 was detectable in WT mice (+/+). Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
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