B-hPD-1/hPD-L1/hCD3E mice
| Strain Name |
C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Cd3etm2(CD3E)Bcgen/Bcgen |
Common Name | B-hPD-1/hPD-L1/hCD3E mice |
| Background | C57BL/6 | Catalog number |
111923 |
|
Related Genes |
PDCD1 (Programmed death-1，as known as PD-1); Cd274 (CD274 antigen); CD3E (CD3E molecule) |
||
模型验证
Protein expression analysis in T cells
Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was detectable in WT mice (+/+). Human PD-1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD3E expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).
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