Strain Name |
C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen ,Tg(RP11-634C1)1Bcgen/Bcgen
|
Common Name | B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice |
Background | C57BL/6 | Catalog number | 111954 |
Related Genes |
PD-1 (Programmed death-1) ; |
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of leukocytes cell subpopulation in lymph node